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021-2023 0623
Ni Bestaresin
Ni Bestaresin 6 High Flow
Ni Bestaresin Mustang
Separation and purification via fixed metal ion affinity chromatography is achieved by interaction between a specific amino acid side chain (mainly histidine) and chelated metal ion. Our Ni series media is suitable for purification of his-tagged protein. Advanced crosslinking process of 6% agarose ensure the high velocity and capacity of the media as well as low limit of Ni2+ leakage, which makes it capatible for large-scale purification of his-tagged protein.
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Buy Hotline: 021-58953301 / 021-2023 0623
| Product | Code no. | Pack size |
| Ni Bestaresin HF | AA200501 | 25ml |
| AA200502 | 100ml | |
| AA200503 | 500ml | |
| AA200504 | 1L | |
| AA200505 | 5L | |
| AA200506 | 10L | |
| AA200507 | 20L | |
| AA200508 | 40L | |
| Ni Bestaresin HP | AA200401 | 25ml |
| AA200402 | 100ml | |
| AA200403 | 500ml | |
| AA200404 | 1L | |
| AA200405 | 5L | |
| AA200406 | 10L | |
| AA200407 | 20L | |
| AA200408 | 40L |
Specification
Matrix
Highly crosslinked 6% agarose
Average Particle Size
Ni Bestaresin 6 High Flow 90μm
Ni Bestaresin Mustang 34μm
Dynamic Bind Capacity (150cm/h):
Approximately 40mg his-tagged protein/ml media
Metal Ion Capacity
Approximately 15μmolNi2+/ml media
Suggested Flow Rate
<150cm/h
Compatibility
Stable in all general Bufffer, reducer, denaturant and detergent
Chemical Stability
40℃, 1week：0.01M HCl，0.1M NaOH；
12h：1M NaOH，70% Acetic acid；
30min：30% isopropanol，1h：2%SDS；
pH Stability
3-12(working)，2-14(CIP, short -term)
Max Pressure
Ni Bestaresin 6 High Flow 1bar(0.1MPa)
Ni Bestaresin Mustang 3bar(0.3MPa)
Storing Solution
20%Ethanol
Storing Temperature
4℃-30℃
應用實例
Media：Ni Bestaresin 6 High Flow
Column：EzFast 5 ml
Sample：His-tagged recombinant protein
Binding Buffer：20mM sodium phosphate，500mM NaCl，
40mM imidazole pH7.4
Elution Buffer：20mM sodium phosphate，500mM NaCl，
500mM imidazole pH7.4
Flow Rate：0.7ml/min
Ni Bestaresin 6 HF purification of His-tagged recombinant protein